Control:Article Title: Herpes simplex virus 1 encodes a STING antagonist that can be therapeutically targeted.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse control IgG Protein Tech Group Cat# B900620 Rabbit control IgG Millipore Cat# 12–370 HRP-conjugated goat-anti mouse IgG Thermo Fisher Scientific Cat# PA1-86717; RRID:AB_933847 HRP-conjugated goat-anti rabbit IgG Thermo Fisher Scientific Cat# SA1-9510; RRID:AB_1086400 Anti-FLAG Sigma-Aldrich Cat# F3165; RRID:AB_259529 Anti-HA ABclonal Cat# AE008; RRID:AB_2770404 Anti-GFP ABclonal Cat# AE012; RRID:AB_2770402 Anti-STING Cell Signaling Technology Cat# 13647; RRID: N/A Anti-TBK1 Cell Signaling Technology Cat# 3504, RRID:AB_2255663 Anti-IRF3 Cell Signaling Technology Cat# 4302, RRID:AB_1904036 Anti-Phosoho-STING(Ser366) Cell Signaling Technology Cat# 82083, RRID: N/A Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: N/A Anti-Phospho-IRF-3 (Ser396) Cell Signaling Technology Cat# 4947, RRID:AB_823547 Anti-a-Tubulin Cell Signaling Technology Cat# 2144; RRID: N/A Anti-UL38 Rabbit polyclonal Antibody Atagenix N/A Bacterial and virus strains Human alphaherpesvirus 1 (HSV-1) strain F Wang et al., 2024 N/A HSV-1UL38D81-90 This paper N/A Chemicals, peptides, and recombinant proteins TAT47-57 (YGRKKRRQRRR) This paper N/A .. LILPELQARIRTYNQHYNNLL) This paper N/A G10L(YGRKKRRQRRRGYLRLILPEL) This paper N/A I10R(YGRKKRRQRRRILPELQARIR) This paper N/A Q10H(YGRKKRRQRRRQARIRTYNQH) This paper N/A L4P(YGRKKRRQRRRLILP) This paper N/A Cy5-L4P(Cy5-YGRKKRRQRRRLILP) This paper N/A Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 Proteinase K-Agarose Sigma Cat# P9290 Protease/phosphatase inhibitor cocktail TargetMol Cat# C0002 DMEM Gibco Cat# A4192101 RPMI 1640 Gibco Cat# 11875119 FBS Gibco Cat# 10099141C 33FLAG peptide Sigma Cat# F4799 c[30-Biotin-16-G(20,5’)pA(30,5’)p] InvivoGen Cat# C 196-001 2030-cGAMP InvivoGen Cat# tlrl-nacga23-02 Streptomycin-penicillin Gibco Cat# 15140122 Low-melting-point agarose Sigma-Aldrich Cat# A9414 Type II collagenase Worthington Cat# LS005273 DNase I Thermo Fisher Scientific Cat# EN0521 Protease inhibitor cocktail MCE Cat# HY-K0010 ECL 4A Biotech Cat# BN01036 RNase inhibitor Promega Cat# N2115 Protein A/G Magnetic Beads MCE Cat# HY-K0202 (Continued on next page) e1 Cell Reports Medicine 6, 102051, April 15, 2025
Virus:Article Title: Herpes simplex virus 1 encodes a STING antagonist that can be therapeutically targeted.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse control IgG Protein Tech Group Cat# B900620 Rabbit control IgG Millipore Cat# 12–370 HRP-conjugated goat-anti mouse IgG Thermo Fisher Scientific Cat# PA1-86717; RRID:AB_933847 HRP-conjugated goat-anti rabbit IgG Thermo Fisher Scientific Cat# SA1-9510; RRID:AB_1086400 Anti-FLAG Sigma-Aldrich Cat# F3165; RRID:AB_259529 Anti-HA ABclonal Cat# AE008; RRID:AB_2770404 Anti-GFP ABclonal Cat# AE012; RRID:AB_2770402 Anti-STING Cell Signaling Technology Cat# 13647; RRID: N/A Anti-TBK1 Cell Signaling Technology Cat# 3504, RRID:AB_2255663 Anti-IRF3 Cell Signaling Technology Cat# 4302, RRID:AB_1904036 Anti-Phosoho-STING(Ser366) Cell Signaling Technology Cat# 82083, RRID: N/A Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: N/A Anti-Phospho-IRF-3 (Ser396) Cell Signaling Technology Cat# 4947, RRID:AB_823547 Anti-a-Tubulin Cell Signaling Technology Cat# 2144; RRID: N/A Anti-UL38 Rabbit polyclonal Antibody Atagenix N/A Bacterial and virus strains Human alphaherpesvirus 1 (HSV-1) strain F Wang et al., 2024 N/A HSV-1UL38D81-90 This paper N/A Chemicals, peptides, and recombinant proteins TAT47-57 (YGRKKRRQRRR) This paper N/A .. LILPELQARIRTYNQHYNNLL) This paper N/A G10L(YGRKKRRQRRRGYLRLILPEL) This paper N/A I10R(YGRKKRRQRRRILPELQARIR) This paper N/A Q10H(YGRKKRRQRRRQARIRTYNQH) This paper N/A L4P(YGRKKRRQRRRLILP) This paper N/A Cy5-L4P(Cy5-YGRKKRRQRRRLILP) This paper N/A Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 Proteinase K-Agarose Sigma Cat# P9290 Protease/phosphatase inhibitor cocktail TargetMol Cat# C0002 DMEM Gibco Cat# A4192101 RPMI 1640 Gibco Cat# 11875119 FBS Gibco Cat# 10099141C 33FLAG peptide Sigma Cat# F4799 c[30-Biotin-16-G(20,5’)pA(30,5’)p] InvivoGen Cat# C 196-001 2030-cGAMP InvivoGen Cat# tlrl-nacga23-02 Streptomycin-penicillin Gibco Cat# 15140122 Low-melting-point agarose Sigma-Aldrich Cat# A9414 Type II collagenase Worthington Cat# LS005273 DNase I Thermo Fisher Scientific Cat# EN0521 Protease inhibitor cocktail MCE Cat# HY-K0010 ECL 4A Biotech Cat# BN01036 RNase inhibitor Promega Cat# N2115 Protein A/G Magnetic Beads MCE Cat# HY-K0202 (Continued on next page) e1 Cell Reports Medicine 6, 102051, April 15, 2025
Recombinant:Article Title: Herpes simplex virus 1 encodes a STING antagonist that can be therapeutically targeted.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse control IgG Protein Tech Group Cat# B900620 Rabbit control IgG Millipore Cat# 12–370 HRP-conjugated goat-anti mouse IgG Thermo Fisher Scientific Cat# PA1-86717; RRID:AB_933847 HRP-conjugated goat-anti rabbit IgG Thermo Fisher Scientific Cat# SA1-9510; RRID:AB_1086400 Anti-FLAG Sigma-Aldrich Cat# F3165; RRID:AB_259529 Anti-HA ABclonal Cat# AE008; RRID:AB_2770404 Anti-GFP ABclonal Cat# AE012; RRID:AB_2770402 Anti-STING Cell Signaling Technology Cat# 13647; RRID: N/A Anti-TBK1 Cell Signaling Technology Cat# 3504, RRID:AB_2255663 Anti-IRF3 Cell Signaling Technology Cat# 4302, RRID:AB_1904036 Anti-Phosoho-STING(Ser366) Cell Signaling Technology Cat# 82083, RRID: N/A Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: N/A Anti-Phospho-IRF-3 (Ser396) Cell Signaling Technology Cat# 4947, RRID:AB_823547 Anti-a-Tubulin Cell Signaling Technology Cat# 2144; RRID: N/A Anti-UL38 Rabbit polyclonal Antibody Atagenix N/A Bacterial and virus strains Human alphaherpesvirus 1 (HSV-1) strain F Wang et al., 2024 N/A HSV-1UL38D81-90 This paper N/A Chemicals, peptides, and recombinant proteins TAT47-57 (YGRKKRRQRRR) This paper N/A .. LILPELQARIRTYNQHYNNLL) This paper N/A G10L(YGRKKRRQRRRGYLRLILPEL) This paper N/A I10R(YGRKKRRQRRRILPELQARIR) This paper N/A Q10H(YGRKKRRQRRRQARIRTYNQH) This paper N/A L4P(YGRKKRRQRRRLILP) This paper N/A Cy5-L4P(Cy5-YGRKKRRQRRRLILP) This paper N/A Cell Counting Kit-8 (CCK-8) Dojindo Cat# CK04 Proteinase K-Agarose Sigma Cat# P9290 Protease/phosphatase inhibitor cocktail TargetMol Cat# C0002 DMEM Gibco Cat# A4192101 RPMI 1640 Gibco Cat# 11875119 FBS Gibco Cat# 10099141C 33FLAG peptide Sigma Cat# F4799 c[30-Biotin-16-G(20,5’)pA(30,5’)p] InvivoGen Cat# C 196-001 2030-cGAMP InvivoGen Cat# tlrl-nacga23-02 Streptomycin-penicillin Gibco Cat# 15140122 Low-melting-point agarose Sigma-Aldrich Cat# A9414 Type II collagenase Worthington Cat# LS005273 DNase I Thermo Fisher Scientific Cat# EN0521 Protease inhibitor cocktail MCE Cat# HY-K0010 ECL 4A Biotech Cat# BN01036 RNase inhibitor Promega Cat# N2115 Protein A/G Magnetic Beads MCE Cat# HY-K0202 (Continued on next page) e1 Cell Reports Medicine 6, 102051, April 15, 2025
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